In the past decade, microfluidic technology has attracted great attention due to the advantage of separating particles of micron and nanoscale. Such processes have a great, but yet not fully explored, potential in biomedicine, pharmacy, food and chemical industry. Such approaches also show high potential in the field of rapid diagnosis, especially the development of point-of-care testing (POCT) methods that are integrated in microfluidic chips. Microfluidic separation of mircroparticles is based on the following principle: particles of different properties such as size, density and shape develop different equilibrium positions in microfluidic devices based on hydrodynamic forces without utilizing external force fields. These particle streaks can then be separated at extremely high efficiency. Among all particle properties, shape is an important marker of cell cycle and indicator of cell state. To develop such microflow based novel methods applicable to cell systems, non-spherical model particles with various well-controllable aspect ratios are highly needed.